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KMID : 0613820090190050684
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Journal of Life Science
2009 Volume.19 No. 5 p.684 ~ p.687
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Genetic Diversity of Xanthomonas arboricola pv. pruni Isolated in Korea
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Park So-Yeon
Lee Young-Sun
Shin Jong-Sub
Koh Young-Jin
Jung Jae-Sung
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Abstract
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Xanthomonas arboricola pv. pruni, the causal agent of bacterial shot holes in stone fruits, was known to have a low population diversity. To investigate the genetic characteristics of X. arboricola pv. pruni isolated in Korea, three strains which have identical 16S rDNA sequences - including type strain (LMG852), Japanese isolate (MAFF301420) and Korean isolate (XWD1) - were analysed based on the nucleotide sequences of three DNA regions and RAPD pattern. No sequence diversity among the three strains was found within the ITS, glnA and atpD gene sequences. However, five of 756 nucleotides of the atpD gene determined (accession number FJ429319) were different from those of the French strain available from the Genbank database. RAPD analyses performed with 40 different arbitrary primers revealed that two strains isolated from Korea and Japan showed similarity in their band patterns distinguished by type strain. These results suggest that Korean and Japanese strains are very close and belong to a population with a low genetic diversity, and might have a different origin from strains found in West Europe.
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KEYWORD
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Xanthomonas arboricola pv. pruni, bacterial shot hole, genetic diversity, RAPD
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